Glutathione Peroxidase Kit

Glutathione Peroxidase kit  play important roles in the protection of organisms from oxidative damage. Glutathione Peroxidase Assay Kit (Colorimetric) is an assay where glutathione peroxidase (GPx) reduces the probe Cumene Hydroperoxide while oxidizing GSH to GSSG. The generated GSSG is reduced to GSH with consumption of NADPH by glutathione reductase (GR). The decrease of NADPH (easily measured at OD=340 nm) is proportional to GPx activity. The assay can be used to measure all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates with a detection sensitivity of ~ 0.5 mU/ml of GPx in samples.

Glutathione peroxidase kit  provides a mechanism for detoxification of peroxides in living cells. This reaction plays a crucial role in protecting cells from damage by free radicals, which are formed by peroxide decomposition. Lipid components of the cell are especially susceptible to reactions with free radicals, resulting in lipid peroxidation. GPx enzymes reduce peroxides to alcohols using glutathione, thus preventing the formation of free radicals.