Cholesterol Assay Kit

The Bioassaysys’s Cholesterol Assay Kit provides a sensitive, rapid, and simple fluorometric method for detecting very low concentrations of cholesterol using a fluorescence microplate reader or fluorometer. The assay measures both free cholesterol and cholesteryl esters. Bioassaysys’s Cholesterol Assay Kit measures the total cholesterol within serum, plasma, lysate, or tissue samples. The assay is based on the enzyme driven reaction that quantifies both cholesterol esters and free cholesterol. Cholesterol esters are hydrolyzed via cholesterol esterase into cholesterol, which is then oxidized by cholesterol oxidase into the ketone cholest-4-en-3-one plus hydrogen peroxide.

Cholesterol Assay Kit provides a simple quantification method of HDL and LDL/VLDL after a convenient separation of HDL from LDL and VLDL (very low-density lipoprotein) in serum samples. In this assay, cholesterol oxidase specifically recognizes free cholesterol and produces a component that will react with probe to generate color (570 nm) and fluorescence (Ex/Em = 538/587 nm). Cholesterol esterase hydrolizes cholesteryl ester into free cholesterol, therefore, cholesterol ester and free cholesterol can be detected separately in the presence and absence of cholesterol esterase in the reactions.