BioAssaysys’s Fatty Acid Assay Kit provides a convenient,
sensitive enzyme-based method for detecting the long-chain free fatty acids
(FA) in various biological samples, such as serum, plasma and other body
fluids, food, growth media, etc. In this assay, FA are converted to their CoA
derivatives (coenzyme A), which are subsequently oxidized, leading to formation
of color/ fluorescence. Fatty acids can then be easily quantified by either
colorimetric or fluorometric .
The Fatty Acid Assay Kit is a simple, colorimetric assay that
quantitatively measures the free fatty acid concentration (non-esterified) in
various samples using a 96-well microtiter plate format. Each kit provides
sufficient reagents to perform up to 100 assays, including blanks, standards
and unknown samples. The kit contains a palmitic acid standard and has a
detection sensitivity limit of ~15 µM. This kit is not suitable for urine or heparin-containing
samples. Fatty acids (C8 and longer) can be quantified with this kit.
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