Catalase Assay Kit

Bioassaysys’s Catalase Assay Kit provides a highly sensitive, simple, direct and HTS-ready assay for measuring catalase activity in biological samples. In the assay, catalase first reacts with H2O2 to produce water and oxygen, the unconverted H2O2 reacts with OxiRed probe to produce a product, which can be measured at 570 nm (Colorimetric method) or at Ex/Em=535/587 nm (fluorometric method). Catalase activity is reversely proportional to the signal. The kit can detect 1 μU of catalase activity in samples.

Catalase Activity Assay involves two reactions. The first reaction is the catalase induced decomposition of hydrogen peroxide H2O2 into water and oxygen. The rate of disintegration of hydrogen peroxide into water and oxygen is proportional to the concentration of catalase (See Reaction 1 in Figure 1). A catalase-containing sample can be incubated in a known amount of hydrogen peroxide. The reaction proceeds for exactly one minute, at which time the catalase is quenched with sodium azide. The remaining hydrogen peroxide in the reaction mixture facilitates the coupling reaction of DHBS and AAP in conjunction with an HRP catalyst (See Reaction 2 in Figure 1). The quinoneimine dye coupling product is measured at 520nm, which correlates to the amount of hydrogen peroxide remaining in the reaction mixture