Aconitase Assay Kit is a highly sensitive, simple, direct
and HTS-ready colorimetric assay for measuring Aconitase activity in biological
samples. In the assay, citrate is converted by aconitase into isocitrate, which
is further processed resulting in a product that converts a nearly colorless
probe into an intensely colored form with a λmax at 450nm.
Aconitase is an iron-sulfur protein containing a [Fe4S4]2+
cluster that catalyzes the stereospecific isomerization of citrate to
isocitrate via cis-aconitate. Whereas exposure of aconitase to oxidants renders
the enzyme inactive, loss of aconitase activity in cells or in biological
samples treated with pro-oxidants has been interpreted as a measure of
oxidative damage. Bioassaysys’s Aconitase Assay Kit provides a simple,
reproducible, and sensitive tool for assaying aconitase from tissue homogenates
or cell lysates. In this assay, citrate is isomerized by aconitase into
isocitrate, which is then converted to α-ketoglutarate in a reaction catalyzed
by isocitric dehydrogenase.
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