Adipolysis Assay Kit

Adipolysis Assay Kit is a quantitative colorimetric/fluorimetric assay for adipolysis.Adipolysis Assay Kit Provides a complete assay for studying the lipolysis of triglycerides from the differenciation of adipocyte like cells to the quantitation of released glycerol. Free glycerol in the cell culture medium is measured by incubation with glycerol kinase, glycerol phosphate oxidase, and horseradish peroxidase in the presence of a colorimetric substrate to generate a chromophore detectable at 540 nm. The amount of glycerol release is correlated with both the amount of stored triglyceride and the degree of lipolysis, making this kit useful for investigators screening compounds affecting either lipid storage or metabolism. The Adipolysis Assay Kit provides enough reagents to assay one 96-well plate of supernatants for glycerol release. The accumulation of lipid droplets can be alternatively assayed with either the Lipid Droplets Fluorescence Assay Kit or the Adipogenesis Assay Kit and glycerol release alone can be assayed using the Glycerol Cell-Based Assay Kit

Adipolysis or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust, and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery. This adipolysis assay kit directly measures glycerol released during adipolysis in cultured cells. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase, and color reactions in one step.