ALT Assay Kit is a colorimetric method for assaying the
activity of ALT.Alanine Aminotransferase (ALT), also known as serum
glutamic-pyruvic transaminase (SGPT), is a pyridoxal-phosphate-dependent enzyme
that catalyzes the reversible transfer of an amino group from alanine to
α-ketoglutarate, generating pyruvate and glutamate. ALT is found primarily in
liver and serum, but occurs in other tissues as well. Hepatocellular injury
often results in an increase of serum ALT levels and serum ALT levels can be
used as a marker for liver injury.
ALT is also a very useful tool for preclinical investigation
of experimental drug formulations and ALT levels are commonly used to monitor
and attenuate the hepatotoxic effects of experimental drugs in rodents.Our kits
can work with a variety of mammalian samples including rats and pig. You might
have to try a few different volumes to make sure the readings are within the
linear range of the std. curve. This kit has a detection limit of 10 mU of
enzyme activity per well.
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