Cytotoxicity Assay Kit

Cytotoxicity Assay Kit is a reliable colorimetric assay to quantitatively measure lactate dehydrogenase (LDH) released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis.Cytotoxicity Assay Kit measures extracellular LDH in culture media using an enzymatic reaction that results in a red formazan product which can be measured spectrophotometrically. Lactate dehydrogenase (LDH) is a cytosolic enzyme that is is an indicator of cellular toxicity. The assay is ideal for high-throughput screening and provides a safe alternative to radioactive cytotoxicity assays. Kit contains substrate mix, assay buffer, 10X lysis buffer, stop solution, and LDH positive control The kit can be used with different cell types for measuring cytoxicity mediated by chemical compounds as well as assaying cell-mediated cytotoxicity.

The Cytotoxicity Assay Kit is a fast and simple method to quantify cytotoxicity/cytolysis based on the measurement of LDH activity released from damaged cells using the 96-well plate format. Thus, the kit can be used in many different in vitro cell systems when damage to the plasma membrane occurs. For example:

• Detection and quantification of cell-mediated cytotoxicity

• Determination of mediator-induced cytolysis

• Determination of the cytotoxic potential of compounds in environmental and medical research, and in the food, cosmetic, and pharmaceutical industries

• Determination of cell death in bioreactors