Glutathione Peroxidase Kit

Glutathione Peroxidase kit  play important roles in the protection of organisms from oxidative damage. Glutathione Peroxidase Assay Kit (Colorimetric) is an assay where glutathione peroxidase (GPx) reduces the probe Cumene Hydroperoxide while oxidizing GSH to GSSG. The generated GSSG is reduced to GSH with consumption of NADPH by glutathione reductase (GR). The decrease of NADPH (easily measured at OD=340 nm) is proportional to GPx activity. The assay can be used to measure all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates with a detection sensitivity of ~ 0.5 mU/ml of GPx in samples.

Glutathione peroxidase kit  provides a mechanism for detoxification of peroxides in living cells. This reaction plays a crucial role in protecting cells from damage by free radicals, which are formed by peroxide decomposition. Lipid components of the cell are especially susceptible to reactions with free radicals, resulting in lipid peroxidation. GPx enzymes reduce peroxides to alcohols using glutathione, thus preventing the formation of free radicals.

Glucose assay Kit

Glucose Assay Kit provides direct measurement of glucose in various biological samples (e.g., serum, plasma, body fluid, food, growth medium, etc.). Glucose Enzyme Mix specifically oxidizes glucose to generate a product which reacts with a dye to generate and fluorescence. The generated color and fluorescence is proportionally to the glucose amount. The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. The kit detects 1-10000 µM glucose samples.

Glucose assay Kit is for the quantitative, enzymatic determination of glucose in food and other materials. Glucose is oxidized to gluconic acid and hydrogen peroxide by glucose oxidase. Hydrogen peroxide reacts with o-dianisidine in the presence of peroxidase to form a colored product. Oxidized o-dianisidine reacts with sulfuric acid to form a more stable colored product. The intensity of the pink color measured at 540 nm is proportional to the original glucose concentration.

Fatty Acid Assay Kit

BioAssaysys’s Fatty Acid Assay Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids (FA) in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In this assay, FA are converted to their CoA derivatives (coenzyme A), which are subsequently oxidized, leading to formation of color/ fluorescence. Fatty acids can then be easily quantified by either colorimetric or fluorometric .

The Fatty Acid Assay Kit is a simple, colorimetric assay that quantitatively measures the free fatty acid concentration (non-esterified) in various samples using a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and unknown samples. The kit contains a palmitic acid standard and has a detection sensitivity limit of ~15 µM. This kit is not suitable for urine or heparin-containing samples. Fatty acids (C8 and longer) can be quantified with this kit.

Cytotoxicity Assay Kit

Cytotoxicity Assay Kit is a reliable colorimetric assay to quantitatively measure lactate dehydrogenase (LDH) released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis.Cytotoxicity Assay Kit measures extracellular LDH in culture media using an enzymatic reaction that results in a red formazan product which can be measured spectrophotometrically. Lactate dehydrogenase (LDH) is a cytosolic enzyme that is is an indicator of cellular toxicity. The assay is ideal for high-throughput screening and provides a safe alternative to radioactive cytotoxicity assays. Kit contains substrate mix, assay buffer, 10X lysis buffer, stop solution, and LDH positive control The kit can be used with different cell types for measuring cytoxicity mediated by chemical compounds as well as assaying cell-mediated cytotoxicity.

The Cytotoxicity Assay Kit is a fast and simple method to quantify cytotoxicity/cytolysis based on the measurement of LDH activity released from damaged cells using the 96-well plate format. Thus, the kit can be used in many different in vitro cell systems when damage to the plasma membrane occurs. For example:

• Detection and quantification of cell-mediated cytotoxicity

• Determination of mediator-induced cytolysis

• Determination of the cytotoxic potential of compounds in environmental and medical research, and in the food, cosmetic, and pharmaceutical industries

• Determination of cell death in bioreactors

Catalase Assay Kit

Bioassaysys’s Catalase Assay Kit provides a highly sensitive, simple, direct and HTS-ready assay for measuring catalase activity in biological samples. In the assay, catalase first reacts with H2O2 to produce water and oxygen, the unconverted H2O2 reacts with OxiRed probe to produce a product, which can be measured at 570 nm (Colorimetric method) or at Ex/Em=535/587 nm (fluorometric method). Catalase activity is reversely proportional to the signal. The kit can detect 1 μU of catalase activity in samples.

Catalase Activity Assay involves two reactions. The first reaction is the catalase induced decomposition of hydrogen peroxide H2O2 into water and oxygen. The rate of disintegration of hydrogen peroxide into water and oxygen is proportional to the concentration of catalase (See Reaction 1 in Figure 1). A catalase-containing sample can be incubated in a known amount of hydrogen peroxide. The reaction proceeds for exactly one minute, at which time the catalase is quenched with sodium azide. The remaining hydrogen peroxide in the reaction mixture facilitates the coupling reaction of DHBS and AAP in conjunction with an HRP catalyst (See Reaction 2 in Figure 1). The quinoneimine dye coupling product is measured at 520nm, which correlates to the amount of hydrogen peroxide remaining in the reaction mixture

Cholesterol Assay Kit

The Bioassaysys’s Cholesterol Assay Kit provides a sensitive, rapid, and simple fluorometric method for detecting very low concentrations of cholesterol using a fluorescence microplate reader or fluorometer. The assay measures both free cholesterol and cholesteryl esters. Bioassaysys’s Cholesterol Assay Kit measures the total cholesterol within serum, plasma, lysate, or tissue samples. The assay is based on the enzyme driven reaction that quantifies both cholesterol esters and free cholesterol. Cholesterol esters are hydrolyzed via cholesterol esterase into cholesterol, which is then oxidized by cholesterol oxidase into the ketone cholest-4-en-3-one plus hydrogen peroxide.

Cholesterol Assay Kit provides a simple quantification method of HDL and LDL/VLDL after a convenient separation of HDL from LDL and VLDL (very low-density lipoprotein) in serum samples. In this assay, cholesterol oxidase specifically recognizes free cholesterol and produces a component that will react with probe to generate color (570 nm) and fluorescence (Ex/Em = 538/587 nm). Cholesterol esterase hydrolizes cholesteryl ester into free cholesterol, therefore, cholesterol ester and free cholesterol can be detected separately in the presence and absence of cholesterol esterase in the reactions.

Caspase Assay Kit

Caspase 3 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (p-NA) after cleavage from the labeled substrate DEVD-pNA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of p-NA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase 3 activity.

Caspase 3 Colorimetric Assay Kit provides the reagents needed for a quick and efficient detection of caspase 3 activity in cell lysates and in purified preparations of caspase 3. Active caspase-3 generates a fluorescent product that is easily quantified.Caspase-3 plays a central role in the execution of apoptosis. Activation of caspase-3 is commonly used as a biomarker for assessment of apoptosis and for understanding mechanisms of apoptosis induction.

Aconitase Assay Kit

Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a λmax at 450nm.

Aconitase is an iron-sulfur protein containing a [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate. Whereas exposure of aconitase to oxidants renders the enzyme inactive, loss of aconitase activity in cells or in biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage. Bioassaysys’s Aconitase Assay Kit provides a simple, reproducible, and sensitive tool for assaying aconitase from tissue homogenates or cell lysates. In this assay, citrate is isomerized by aconitase into isocitrate, which is then converted to α-ketoglutarate in a reaction catalyzed by isocitric dehydrogenase. 

ALT Assay Kit

ALT Assay Kit is a colorimetric method for assaying the activity of ALT.Alanine Aminotransferase (ALT), also known as serum glutamic-pyruvic transaminase (SGPT), is a pyridoxal-phosphate-dependent enzyme that catalyzes the reversible transfer of an amino group from alanine to α-ketoglutarate, generating pyruvate and glutamate. ALT is found primarily in liver and serum, but occurs in other tissues as well. Hepatocellular injury often results in an increase of serum ALT levels and serum ALT levels can be used as a marker for liver injury.

ALT is also a very useful tool for preclinical investigation of experimental drug formulations and ALT levels are commonly used to monitor and attenuate the hepatotoxic effects of experimental drugs in rodents.Our kits can work with a variety of mammalian samples including rats and pig. You might have to try a few different volumes to make sure the readings are within the linear range of the std. curve. This kit has a detection limit of 10 mU of enzyme activity per well.

Adipolysis Assay Kit

Adipolysis Assay Kit is a quantitative colorimetric/fluorimetric assay for adipolysis.Adipolysis Assay Kit Provides a complete assay for studying the lipolysis of triglycerides from the differenciation of adipocyte like cells to the quantitation of released glycerol. Free glycerol in the cell culture medium is measured by incubation with glycerol kinase, glycerol phosphate oxidase, and horseradish peroxidase in the presence of a colorimetric substrate to generate a chromophore detectable at 540 nm. The amount of glycerol release is correlated with both the amount of stored triglyceride and the degree of lipolysis, making this kit useful for investigators screening compounds affecting either lipid storage or metabolism. The Adipolysis Assay Kit provides enough reagents to assay one 96-well plate of supernatants for glycerol release. The accumulation of lipid droplets can be alternatively assayed with either the Lipid Droplets Fluorescence Assay Kit or the Adipogenesis Assay Kit and glycerol release alone can be assayed using the Glycerol Cell-Based Assay Kit

Adipolysis or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust, and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery. This adipolysis assay kit directly measures glycerol released during adipolysis in cultured cells. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase, and color reactions in one step.

Acetylcholine Assay Kit

Acetylcholine is a polyatomic neurotransmitter that is produced in acetyl cholinergic neurons. Acetylcholine Assay Kit provides an ultra sensitive method for detecting acetyl cholinesterase (AChE) activity in a fluorescence micro plate reader. Acetylcholine Assay Kit is a quantitative colorimetric acetylcholine determination. It is one of many neurotransmitters within the autonomic nervous system and the only neurotransmitter in the motor function of the somatic nervous system.

 Acetylcholine works within the peripheral and central nervous systems within many organisms, including humans. It is manufactured via choline acetyltransferase from acetyl-CoA and choline.

Bioassaysys’s   Acetylcholine Assay Kit is based on the enzyme driven reaction that will detect acetylcholine via acetyl cholinesterase enzyme and choline oxidase. First, acetyl cholinesterase hydrolyzes acetylcholine into choline and acetic acid. Choline is then oxidized by choline oxidase to produce hydrogen peroxide. The hydrogen peroxide is then detected with a highly specific colorimetric probe